mouse cd8 til micro beads  (Miltenyi Biotec)

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Stress induces corticosterone-mediated CD8 + T cell exhaustion to promote non-small cell lung cancer

doi: 10.1007/s00262-025-04179-w

Figure Lengend Snippet: Stress impairs NSCLC immunosurveillance. a Flow cytometric logic diagram of tumor tissue. Flow cytometric analysis of the proportions of b tumor CD45 + , c lymphoid and myeloid, d macrophage, e NK cell, f dendritic cell, g CD3 + T cell, h CD4 + and CD8 + T cell at the tumor endpoint between the control (Con) and acute restraint (AR) mice (n = 4). Data are represented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 by an unpaired Student’s t-test

Article Snippet: CD8 (TIL) Micro Beads (130–116-478, Miltenyi Biotec) were used to sort CD8 + T cells from tumor tissues according to the instructions and verify their purity through flow cytometry.

Techniques: Control

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Stress induces corticosterone-mediated CD8 + T cell exhaustion to promote non-small cell lung cancer

doi: 10.1007/s00262-025-04179-w

Figure Lengend Snippet: Stress induces CD8 + T cell exhaustion. Flow cytometric analysis of the proportions of a tumor Ki67 + CD8 + , b PD-1 + CD8 + and c TIM-3 + CD8 + at the tumor endpoint between the control (Con) and acute restraint (AR) mice (n = 4). d – e Immunofluorescence analysis of the expression levels of PD-1, TIM-3 and CD8a in tumor tissues (30x). Data are represented as the mean ± standard error of the mean (SEM). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by an unpaired Student’s t-test

Article Snippet: CD8 (TIL) Micro Beads (130–116-478, Miltenyi Biotec) were used to sort CD8 + T cells from tumor tissues according to the instructions and verify their purity through flow cytometry.

Techniques: Control, Immunofluorescence, Expressing

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Stress induces corticosterone-mediated CD8 + T cell exhaustion to promote non-small cell lung cancer

doi: 10.1007/s00262-025-04179-w

Figure Lengend Snippet: Stress elevates corticosterone in blood and glucocorticoid receptor (GR) in tumor. The concentration of a adrenocorticotropic hormone (ACTH), b corticosterone (COR), c adrenaline and d norepinephrine were tested by ELISA at the tumor endpoint between the control (Con) and acute restraint (AR) mice (n = 6). (e-f) Correlation analysis between ACTH and COR with LDBT and OFT, respectively. (g-j) Correlation analysis between tumor volumes with concentration of ACTH, COR, norepinephrine and adrenaline, respectively. k Immunofluorescence analysis of the expression levels of GR and CD8a in tumor tissues and the white arrows point to CD8 + T cells with high expression of GR (50x). l Flow cytometric analysis of the expression of GR in CD8 + T cells of the control (Con) and corticosterone treatment (COR) mice (n = 3). Data are represented as the mean ± standard error of the mean (SEM). * p < 0.05, ** p < 0.01, *** p < 0.001 by an unpaired Student’s t-test or Pearson analysis

Article Snippet: CD8 (TIL) Micro Beads (130–116-478, Miltenyi Biotec) were used to sort CD8 + T cells from tumor tissues according to the instructions and verify their purity through flow cytometry.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Control, Immunofluorescence, Expressing

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Stress induces corticosterone-mediated CD8 + T cell exhaustion to promote non-small cell lung cancer

doi: 10.1007/s00262-025-04179-w

Figure Lengend Snippet: Corticosterone (COR) promotes CD8 + T cell exhaustion and NSCLC growth. a The LLC tumor growth curves and the endpoint tumor weights were displayed of control (Con) and corticosterone treatment (COR) mice (n = 5). (b-d) Flow cytometric analysis of the proportions of PD-1 + and LAG3 + , granzyme B + and IFN-γ + of CD8 + T cells of tumor tissues from Con and COR mice (n = 4). (e-f) The LLC tumor growth curves, the endpoint tumor weights and tumor sizes were showed of acute restraint (AR+PBS) and acute restraint+RU486 treatment (AR+RU486) mice (n = 6). g CCK-8 detection of CD8 + T cell viability after 24 hours of treatment with different concentrations of COR. Flow cytometric analysis of the proportions of h IFN-γ + , i granzyme B + , j LAG3 + , k PD-1 + and l TIM-3 + of CD8 + T cells among DMSO, COR and COR+RU486 treatment groups (n = 3). Data are represented as the mean ± standard error of the mean (SEM). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by an unpaired Student’s T-test or one-way ANOVA

Article Snippet: CD8 (TIL) Micro Beads (130–116-478, Miltenyi Biotec) were used to sort CD8 + T cells from tumor tissues according to the instructions and verify their purity through flow cytometry.

Techniques: Control, CCK-8 Assay

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Stress induces corticosterone-mediated CD8 + T cell exhaustion to promote non-small cell lung cancer

doi: 10.1007/s00262-025-04179-w

Figure Lengend Snippet: Smart RNA sequencing reveals the mechanism of stress regulated CD8 + T cell function. a Isolation process of CD8 + T cells from mice tumors. b Differential genes of CD8 + T cells in tumors of the control (Con) and acute stress (AR) mice (n = 3) (|Log 2 (Fold change)| ≥ 1 and p < 0.05). c KEGG analysis of differential genes of CD8 + T cells and the most significant 20 enriched pathways are displayed ( p < 0.05). d GSEA analysis of differential genes of CD8 + T cells and the most significant 20 enriched pathways are displayed ( p < 0.05). Detect the mRNA expression levels of Plcg1 e , Prkcq f , Tec g , Grap2 h , Rasgrp1 i , Ppp3cc j , Mapk11 k and Mapk14 l in CD8 + T cells among DMSO, COR and COR+RU486 treatment groups using qRT-PCR (n = 3). Data are represented as the mean ± standard error of the mean (SEM). * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 by one-way ANOVA e – l

Article Snippet: CD8 (TIL) Micro Beads (130–116-478, Miltenyi Biotec) were used to sort CD8 + T cells from tumor tissues according to the instructions and verify their purity through flow cytometry.

Techniques: RNA Sequencing, Cell Function Assay, Isolation, Control, Expressing, Quantitative RT-PCR

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Stress induces corticosterone-mediated CD8 + T cell exhaustion to promote non-small cell lung cancer

doi: 10.1007/s00262-025-04179-w

Figure Lengend Snippet: GRAP2 involves CD8 + T cell exhaustion in NSCLC. a UMAP plot in NSCLC_GSE149655 scRNA-seq dataset from TISCH2 database. The CD8 Tex mainly consisted of cells from cluster 4 and cluster 13. GRAP2 expresses highly in both clusters CD8 Tex . b UMAP plot in NSCLC_EMTAB6149 scRNA-seq dataset from TISCH2 database. The CD8 Tex mainly consisted of cells from cluster 0, cluster 8 and cluster 12. GRAP2 expresses highly in all three cluster CD8 Tex . (c-f) Correlation analysis of gene expression levels between GRAP2 and PDCD1 c , LAG3 d , HAVCR2 e and TIGIT f of NSCLC patients through TCGA database. g Hazard ratio (HR) of impact of GRAP2 expression levels in CD8 Tex on the survival of different cancer patients. The high expression of GRAP2 in CD8 Tex significantly increases the risk of shortened survival in lung adenocarcinoma patients ( p < 0.05). h Summary of the mechanism of stress promoting CD8 + T cell exhaustion in NSCLC

Article Snippet: CD8 (TIL) Micro Beads (130–116-478, Miltenyi Biotec) were used to sort CD8 + T cells from tumor tissues according to the instructions and verify their purity through flow cytometry.

Techniques: Gene Expression, Expressing